The S 10 technique is the standard technique in Plastination. Specimen Impregnation with S 10 results in opaque, more or less flexible, and natural looking specimens. The procedure consists of the four main steps of Plastination, besides the specimen preparation and dissection before.
FIXATIONFixation can be achieved by all usual fixatives as formaldehyde solution, Kayserling solution etc. Hollow organs must be dilated during fixation as well as during dehydration and gas curing.
Dehydration removes the specimen fluid, as well as some fat. In this step tissue fluid is replaced with an organic solvent. Either alcohol or acetone may be used as a dehydrant for Plastination. Acetone is used in most cases because acetone also serves as the intermediary solvent during the next step - forced impregnation. To minimize specimen shrinkage, dehydration is done in cold (- 15 ° C to 25 ° C) acetone. If the removal of fat is also desired the dehydrated specimen must be kept in acetone at room temperature for some time. An acetone amount of 10 times the specimen weight is best for good results. Dehydration is finished when the water content is less than 1 %.
Equipment: deep freezer (explosion proof or motor and compressor removed and placed in a different room), acetonometer (to measure the content of water).
GAS - CURING (HARDENING)Finally the polymer inside the specimen has to be cured (hardened). This is achieved by exposing the impregnated specimen to a gaseous hardener (BIODUR® S 6). S 6 is a liquid that vaporizes at room temperature. The impregnated specimen and a bowl filled with S 6 is placed in an tightly closed chamber for several weeks. To keep the environment for curing dehumidified a bowl with a desiccant (e.g. calcium chloride) is also placed in the curing chamber. To enhance the curing procedure air may be bubbled through the fluid S 6. For complete curing inside the specimen the specimen should be kept in a plastic bag for several weeks.
Equipment:plastic box, stretch foil, membrane (aquarium) pump.